Application
Western blot analysis of PARP1 (arrow) using rabbit polyclonal PARP1 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PARP1 gene (Lane 2) (Origene Technologies).Application
PARP1 Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the PARP1 antibody detected the PARP1 protein (arrow).Application
Confocal immunofluorescent analysis of PARP1 Antibody (N-term) with Hela cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
PARP1 Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | PARP1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PARP1; ADPRT; PPOL; Poly [ADP-ribose] polymerase 1; ADP-ribosyltransferase diphtheria toxin-like 1; NAD(+) ADP-ribosyltransferase 1; Poly[ADP-ribose] synthase 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIFFC |
| WB:1:50~100 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ADPRT PPOL |
|---|---|
| Alternative Names | PARP1 ADPRT PPOL Poly [ADP-ribose] polymerase 1 ADP-ribosyltransferase diphtheria toxin-like 1 NAD(+) ADP-ribosyltransferase 1 Poly[ADP-ribose] synthase 1 |
| Molecular Weight(MW) | 113084 Da |
| Function | Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP- ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production |
| Tissue Specificity | This PARP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 191~221 amino acids from the N-term of human PARP1. |
| Cellular Localization | Nucleus. Nucleus, nucleolus. |
| Entrez Gene | 142 |
|---|
Application
Western blot analysis of PARP1 (arrow) using rabbit polyclonal PARP1 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PARP1 gene (Lane 2) (Origene Technologies).
Application
PARP1 Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the PARP1 antibody detected the PARP1 protein (arrow).
Application
Confocal immunofluorescent analysis of PARP1 Antibody (N-term) with Hela cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
PARP1 Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
