Application
Western blot analysis of PBX1 (arrow) using rabbit polyclonal PBX1 Antibody (Human N-term) .293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PBX1 gene (Lane 2) (Origene Technologies).Application
Confocal immunofluorescent analysis of PBX1 Antibody (N-term)(Cat#AP7285a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).| Product Name | PBX1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PBX1; PRL; Pre-B-cell leukemia transcription factor 1; Homeobox protein PBX1; Homeobox protein PRL |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:50~100 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PRL |
|---|---|
| Alternative Names | PBX1 PRL Pre-B-cell leukemia transcription factor 1 Homeobox protein PBX1 Homeobox protein PRL |
| Molecular Weight(MW) | 46626 Da |
| Function | Binds the sequence 5'-ATCAATCAA-3'. Acts as a transcriptional activator of PF4 in complex with MEIS1. Converted into a potent transcriptional activator by the (1;19) translocation. May have a role in steroidogenesis and, subsequently, sexual development and differentiation. Isoform PBX1b as part of a PDX1:PBX1b:MEIS2b complex in pancreatic acinar cells is involved in the transcriptional activation of the ELA1 enhancer; the complex binds to the enhancer B element and cooperates with the transcription factor 1 complex (PTF1) bound to the enhancer A element. Probably in complex with MEIS2, is involved in transcriptional regulation by KLF4 |
| Tissue Specificity | Expressed in all tissues except in cells of the B and T lineage |
| Cellular Localization | Nucleus (By similarity). |
| Entrez Gene | 5087 |
|---|
Application
Western blot analysis of PBX1 (arrow) using rabbit polyclonal PBX1 Antibody (Human N-term) .293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PBX1 gene (Lane 2) (Origene Technologies).
Application
Confocal immunofluorescent analysis of PBX1 Antibody (N-term)(Cat#AP7285a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:50~100 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
