Application
PCTK1 N-term western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the PCTK1 antibody detected the PCTK1 protein (arrow).Application
PCTK1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PCTK1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
PCTK1 Antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | PCTK1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CDK16; PCTK1; Cyclin-dependent kinase 16; Cell division protein kinase 16; PCTAIRE-motif protein kinase 1; Serine/threonine-protein kinase PCTAIRE-1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PCTAIRE1 PCTK1 |
|---|---|
| Alternative Names | CDK16 PCTK1 Cyclin-dependent kinase 16 Cell division protein kinase 16 PCTAIRE-motif protein kinase 1 Serine/threonine-protein kinase PCTAIRE-1 |
| Molecular Weight(MW) | 55716 Da |
| Function | Protein kinase that plays a role in vesicle-mediated transport processes and exocytosis. Regulates GH1 release by brain neurons. Phosphorylates NSF, and thereby regulates NSF oligomerization. Required for normal spermatogenesis. Regulates neuron differentiation and dendrite development (By similarity) Plays a role in the regulation of insulin secretion in response to changes in blood glucose levels. Can phosphorylate CCNY at 'Ser- 336' (in vitro) |
| Tissue Specificity | Detected in pancreas islets (at protein level). Detected in brain and pancreas |
| Cellular Localization | Cytoplasm. Cytoplasmic vesicle, secretory vesicle. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction, synapse, synaptosome (By similarity) Note=Colocalizes with insulin in pancreas islets. Recruited to the cell membrane by CCNY |
| Entrez Gene | 5127 |
|---|---|
| Nucleotide Accession | NP_001163931.1 NP_148978.2 |
Application
PCTK1 N-term western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the PCTK1 antibody detected the PCTK1 protein (arrow).
Application
PCTK1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PCTK1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
PCTK1 Antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
