Application
Western blot analysis of PDK1 (arrow) using rabbit polyclonal hPDK1-G14 . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PDK1 gene (Lane 2) (Origene Technologies).| Product Name | PDK1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PDK1; PDHK1; [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial; Pyruvate dehydrogenase kinase isoform 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PDHK1 |
|---|---|
| Alternative Names | PDK1 PDHK1 [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1 mitochondrial Pyruvate dehydrogenase kinase isoform 1 |
| Molecular Weight(MW) | 49244 Da |
| Function | Serine/threonine kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia. Protects cells against apoptosis in response to hypoxia and oxidative stress |
| Tissue Specificity | Expressed predominantly in the heart. Detected at lower levels in liver, skeletal muscle and pancreas |
| Cellular Localization | Mitochondrion matrix. |
| Entrez Gene | 5163 |
|---|
Application
Western blot analysis of PDK1 (arrow) using rabbit polyclonal hPDK1-G14 . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PDK1 gene (Lane 2) (Origene Technologies).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
