Application
Western blot analysis of PDK4 Antibody (E265) in CEM cell line lysates (35ug/lane). PDK4 (arrow) was detected using the purified Pab (1:60 dilution).Application
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with PDK4-E265, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | PDK4 Antibody (E265) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PDK4; PDHK4; [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4, mitochondrial; Pyruvate dehydrogenase kinase isoform 4 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PDHK4 |
|---|---|
| Alternative Names | PDK4 PDHK4 [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4 mitochondrial Pyruvate dehydrogenase kinase isoform 4 |
| Molecular Weight(MW) | 46469 Da |
| Function | Serine/threonine kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Inhibition of pyruvate dehydrogenase decreases glucose utilization and increases fat metabolism in response to prolonged fasting and starvation Plays an important role in maintaining normal blood glucose levels under starvation, and is involved in the insulin signaling cascade. Via its regulation of pyruvate dehydrogenase activity, plays an important role in maintaining normal blood pH and in preventing the accumulation of ketone bodies under starvation. In the fed state, mediates cellular responses to glucose levels and to a high-fat diet. Regulates both fatty acid oxidation and de novo fatty acid biosynthesis. Plays a role in the generation of reactive oxygen species. Protects detached epithelial cells against anoikis. Plays a role in cell proliferation via its role in regulating carbohydrate and fatty acid metabolism |
| Tissue Specificity | Ubiquitous; highest levels of expression in heart and skeletal muscle |
| Cellular Localization | Mitochondrion matrix. |
| Entrez Gene | 5166 |
|---|
Application
Western blot analysis of PDK4 Antibody (E265) in CEM cell line lysates (35ug/lane). PDK4 (arrow) was detected using the purified Pab (1:60 dilution).
Application
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with PDK4-E265, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
