Application
The anti-PDX1 Pab is used in Western blot to detect PDX1 in mouse heart tissue lysate.Application
Western blot analysis of PDX1 (arrow) using rabbit polyclonal PDX1 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PDX1 gene (Lane 2) (Origene Technologies).Application
PDX1 Antibody (R444) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the PDX1 antibody detected the PDX1 protein (arrow).| Product Name | PDX1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PDHX; PDX1; Pyruvate dehydrogenase protein X component, mitochondrial; Dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenase complex; E3-binding protein; Lipoyl-containing pyruvate dehydrogenase complex component X; proX |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PDX1 |
|---|---|
| Alternative Names | PDHX PDX1 Pyruvate dehydrogenase protein X component mitochondrial Dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenase complex E3-binding protein Lipoyl-containing pyruvate dehydrogenase complex component X proX |
| Molecular Weight(MW) | 54122 Da |
| Function | Required for anchoring dihydrolipoamide dehydrogenase (E3) to the dihydrolipoamide transacetylase (E2) core of the pyruvate dehydrogenase complexes of eukaryotes. This specific binding is essential for a functional PDH complex |
| Tissue Specificity | This PDX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 437~466 amino acids from selected from the C-terminal region of human PDX1. |
| Cellular Localization | Mitochondrion matrix. |
| Entrez Gene | 8050 |
|---|
Application
The anti-PDX1 Pab is used in Western blot to detect PDX1 in mouse heart tissue lysate.
Application
Western blot analysis of PDX1 (arrow) using rabbit polyclonal PDX1 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PDX1 gene (Lane 2) (Origene Technologies).
Application
PDX1 Antibody (R444) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the PDX1 antibody detected the PDX1 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
