Application
Western blot analysis of hPGK1-G132 in Hela(lane 1), HepG2(lane 2) cell line lysates (35ug/lane). PGK1 (arrow) was detected using the purified Pab.Application
Western blot analysis of PGK1 (arrow) using rabbit polyclonal PGK1 Antibody (G132) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PGK1 gene.Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PGK1 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of PGK1 Antibody (Center)(Cat#AP7094b) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).Application
PGK1 Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | PGK1 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PGK1; PGKA; Phosphoglycerate kinase 1; Cell migration-inducing gene 10 protein; Primer recognition protein 2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIFFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PGKA |
|---|---|
| Alternative Names | PGK1 PGKA Phosphoglycerate kinase 1 Cell migration-inducing gene 10 protein Primer recognition protein 2 |
| Molecular Weight(MW) | 44615 Da |
| Function | In addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein) |
| Tissue Specificity | This PGK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 124~153 amino acids from the center region of human PGK1. |
| Cellular Localization | Cytoplasm. |
| Entrez Gene | 5230 |
|---|
Application
Western blot analysis of hPGK1-G132 in Hela(lane 1), HepG2(lane 2) cell line lysates (35ug/lane). PGK1 (arrow) was detected using the purified Pab.
Application
Western blot analysis of PGK1 (arrow) using rabbit polyclonal PGK1 Antibody (G132) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PGK1 gene.
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PGK1 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of PGK1 Antibody (Center)(Cat#AP7094b) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Application
PGK1 Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
