Application
Western blot analysis on (A) A549, (B) HeLa and (C) MCF-7 cell lysates using anti-PHD1 RabMAb (Cat. #AJ1601a).Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-PHD1 RabMAb (Cat. #AJ1601a).Application
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-PHD1 RabMAb (Cat. #AJ1601a).| Product Name | PHD1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR2746 |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:1000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | EIT6 |
|---|---|
| Molecular Weight(MW) | 43650 Da |
| Function | Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF2A. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. EGLN2 is involved in regulating hypoxia tolerance and apoptosis in cardiac and skeletal muscle. Also regulates susceptibility to normoxic oxidative neuronal death |
| Tissue Specificity | Expressed in adult and fetal heart, brain, liver, lung, skeletal muscle, and kidney. Also expressed in testis and placenta. Highest levels in adult brain, placenta, lung, kidney, and testis. Expressed in hormone responsive tissues, including normal and cancerous mammary, ovarian and prostate epithelium |
| Cellular Localization | Nucleus. |
| Entrez Gene | 112398 |
|---|
Application
Western blot analysis on (A) A549, (B) HeLa and (C) MCF-7 cell lysates using anti-PHD1 RabMAb (Cat. #AJ1601a).
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-PHD1 RabMAb (Cat. #AJ1601a).
Application
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-PHD1 RabMAb (Cat. #AJ1601a).| Application Notes | WB:1:1000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
