Application
The anti-Phospho-Bad-S118 Pab is used in Western blot to detect Phospho-Bad-S118 in HL60 tissue lysateApplication
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Product Name | Phospho-Bad-S118 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-S118 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | BBC6, BCL2L8 |
|---|---|
| Alternative Names | BAD BBC6 BCL2L8 Bcl2 antagonist of cell death Bcl-2-binding component 6 Bcl-2-like protein 8 Bcl-XL/Bcl-2-associated death promoter |
| Molecular Weight(MW) | 18392 Da |
| Function | Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways |
| Tissue Specificity | Expressed in a wide variety of tissues. |
| Cellular Localization | Mitochondrion outer membrane. Cytoplasm. Note=Upon phosphorylation, locates to the cytoplasm |
| Entrez Gene | 572 |
|---|
Application
The anti-Phospho-Bad-S118 Pab is used in Western blot to detect Phospho-Bad-S118 in HL60 tissue lysate
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
