Application
Dot blot analysis of anti-CCNB1-S126 Phospho-specific Pab on nitrocellulose membrane. 50ng of nonphospho-peptide or phospho-peptide were adsorbed on their respective dots. Antibody working concentration was 0.5ug per ml.Application
he anti-CCNB1-S126 Pab is used in Western blot for detection in Ramos tissue lysate.Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Product Name | Phospho-CCNB1-S126 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-S126 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | DBWBIHC |
| DB:1:100~500 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CCNB |
|---|---|
| Alternative Names | CCNB1 CCNB G2/mitotic-specific cyclin-B1 |
| Molecular Weight(MW) | 48337 Da |
| Function | Essential for the control of the cell cycle at the G2/M (mitosis) transition |
| Tissue Specificity | This Phospho-CCNB1-S126 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S126 of human CCNB1. |
| Cellular Localization | Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, centrosome |
| Entrez Gene | 891 |
|---|
Application
Dot blot analysis of anti-CCNB1-S126 Phospho-specific Pab on nitrocellulose membrane. 50ng of nonphospho-peptide or phospho-peptide were adsorbed on their respective dots. Antibody working concentration was 0.5ug per ml.
Application
he anti-CCNB1-S126 Pab is used in Western blot for detection in Ramos tissue lysate.
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Application Notes | DB:1:100~500 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
