Application
Dot blot analysis of anti-Phospho-Cyclin E1-T395 Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.Application
The anti-Phospho-Cyclin E1-T395 Pab is used in Western blot to detect Phospho-Cyclin E1-T395 in Hela tissue lysate.Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Product Name | Phospho-CCNE1-pT395 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-T395 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | DBWBIHC |
| DB:1:500 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CCNE |
|---|---|
| Alternative Names | CCNE1 CCNE G1/S-specific cyclin-E1 |
| Molecular Weight(MW) | 47077 Da |
| Function | Essential for the control of the cell cycle at the G1/S (start) transition |
| Tissue Specificity | Highly expressed in testis and placenta. Low levels in bronchial epithelial cells |
| Cellular Localization | Nucleus. |
| Entrez Gene | 898 |
|---|
Application
Dot blot analysis of anti-Phospho-Cyclin E1-T395 Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
Application
The anti-Phospho-Cyclin E1-T395 Pab is used in Western blot to detect Phospho-Cyclin E1-T395 in Hela tissue lysate.
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Application Notes | DB:1:500 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
