Application
The anti-phospho-CDC25B-S353 Pab is used in Western blot to detect phospho-CDC25B-S353 in HEPG2 tissue lysateApplication
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma| Product Name | Phospho-CDC25B-S353 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-S353 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CDC25HU2 |
|---|---|
| Alternative Names | CDC25B CDC25HU2 M-phase inducer phosphatase 2 Dual specificity phosphatase Cdc25B |
| Molecular Weight(MW) | 64987 Da |
| Function | Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity |
| Tissue Specificity | This Phospho-CDC25B-S353 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S353 of human CDC25B. |
| Cellular Localization | Cytoplasm, cytoskeleton, centrosome. Cytoplasm, cytoskeleton, spindle pole |
| Entrez Gene | 994 |
|---|
Application
The anti-phospho-CDC25B-S353 Pab is used in Western blot to detect phospho-CDC25B-S353 in HEPG2 tissue lysate
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
