Dot blot analysis of anti-Phospho-ENT1(Slc29a1)-pS254 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
SLC29A1 ENT1 Equilibrative nucleoside transporter 1 Equilibrative nitrobenzylmercaptopurine riboside-sensitive nucleoside transporter Nucleoside transporter es-type Solute carrier family 29 member 1
Molecular Weight(MW)
50219 Da
Function
Mediates both influx and efflux of nucleosides across the membrane (equilibrative transporter). It is sensitive (ES) to low concentrations of the inhibitor nitrobenzylmercaptopurine riboside (NBMPR) and is sodium-independent. It has a higher affinity for adenosine. Inhibited by dipyridamole and dilazep (anticancer chemotherapeutics drugs)
Tissue Specificity
Expressed in heart, brain, mammary gland, erythrocytes and placenta, and also in fetal liver and spleen
Cellular Localization
Basolateral cell membrane; Multi-pass membrane protein. Apical cell membrane; Multi-pass membrane protein. Note=Predominantly localized in the basolateral membrane in polarized MDCK cells
Dot blot analysis of anti-Phospho-ENT1(Slc29a1)-pS254 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
Application Notes
DB:0.388888888889
Additional Information
Form
Liquid
Storage Instructions
For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification.