Application
A. Western blot analysis on serum starved (ss)A431 cell lysate using anti-ERK1(pT202/pY204) /ERK2(pT185/pY187) RabMAb (Cat. #AJ1602a); dilution 1:500Application
B. Immunohistochemical analysis of paraffin-embedded human thyroid gland cancer using anti- ERK1(pT202/pY204) /ERK2(pT185/pY187) RabMAb (Cat. #AJ1602a)| Product Name | Phospho-Erk1 (pT202/pY204) / Erk2 (pT185/pY187) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-T185/p-Y187 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | E337 |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:5000~10000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ERK1, PRKM3 |
|---|---|
| Alternative Names | MAPK3 ERK1 PRKM3 Mitogen-activated protein kinase 3 p44-ERK1 ERT2 p44-MAPK Microtubule-associated protein 2 kinase |
| Molecular Weight(MW) | 43136 Da |
| Function | Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, FRS2 or GRB10) Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade |
| Tissue Specificity | A synthetic phospho-peptide corresponding to residues surrounding Thr202 and Tyr204 of human ERK1 was used as immunogen. The antibody only detects ERK1 phosphorylated on Threonine 202 and Tyrosine 204, or ERK2 phosphorylated on Threonine 185 and Tyrosine 187. |
| Cellular Localization | Cytoplasm. Nucleus. Note=Autophosphorylation at Thr-207 promotes nuclear localization |
| Entrez Gene | 5595 |
|---|
Application
A. Western blot analysis on serum starved (ss)A431 cell lysate using anti-ERK1(pT202/pY204) /ERK2(pT185/pY187) RabMAb (Cat. #AJ1602a); dilution 1:500
Application
B. Immunohistochemical analysis of paraffin-embedded human thyroid gland cancer using anti- ERK1(pT202/pY204) /ERK2(pT185/pY187) RabMAb (Cat. #AJ1602a)| Application Notes | WB:1:5000~10000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
