Application
Western blot analysis of extract from HeLa cells usingHistone H3.1 Antibody (S10) (#AE1016c, Lane 1 and 2)and Phospho-Histone H3.1-S10 Antibody (#AE1016d,Lane 3 and 4).Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Phospho-Histone H3.1-S10 Antibody (#AE1016d)(left) or the same antibody preincubated with blocking peptide(right).Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear dot staining using Phospho-Histone H3.1-S10 Antibody (#AE1016d).| Product Name | Phospho-Histone H3.1-S10 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-S10 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:500~1:1000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | H3FA |
|---|---|
| Alternative Names | Histone H3/l Histone H3/k Histone H3/j Histone H3/i Histone H3/h Histone H3/f Histone H3/d Histone H3/c Histone H3/b Histone H3/a Histone H3.1 |
| Molecular Weight(MW) | 15404 Da |
| Function | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling |
| Tissue Specificity | The antibody was affinity-purified from rabbit antiserum using epitope-specific phosphopeptide column, and the antibody against non-phosphopeptide was removed using non-phosphopeptide column corresponding to the phosphorylation site. |
| Cellular Localization | Nucleus. Chromosome. |
| Entrez Gene | 8968 |
|---|
Application
Western blot analysis of extract from HeLa cells usingHistone H3.1 Antibody (S10) (#AE1016c, Lane 1 and 2)and Phospho-Histone H3.1-S10 Antibody (#AE1016d,Lane 3 and 4).
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Phospho-Histone H3.1-S10 Antibody (#AE1016d)(left) or the same antibody preincubated with blocking peptide(right).
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear dot staining using Phospho-Histone H3.1-S10 Antibody (#AE1016d).| Application Notes | WB:1:500~1:1000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | affinity Purified IgG, in PBS, 0.02% sodium azide and 50% glycerol. |
