Application
Dot blot analysis of anti-MAP2K2-pS226 Phospho-specific Pab (RB12777) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.| Product Name | Phospho-MAP2K2-S226 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-S226 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | DB |
| DB:1:500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MEK2, MKK2, PRKMK2 |
|---|---|
| Alternative Names | MAP2K2 MEK2 MKK2 PRKMK2 Dual specificity mitogen-activated protein kinase kinase 2 ERK activator kinase 2 MAPK/ERK kinase 2 |
| Molecular Weight(MW) | 44424 Da |
| Function | Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases (By similarity) |
| Tissue Specificity | This Phospho-MAP2K2-S226 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S226 of human MAP2K2. |
| Entrez Gene | 5605 |
|---|
Application
Dot blot analysis of anti-MAP2K2-pS226 Phospho-specific Pab (RB12777) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.| Application Notes | DB:1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified rabbit polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with the control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
