Application
Dot blot analysis of Phospho-MAP2K5-S142 polyclonal antibody (Cat# AP3343a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentration was 0.5ug per ml. P-Pab: phospho-antibody; P-Peptide: phospho-peptide; NP-Peptide: non-phospho-peptide.| Product Name | Phospho-MAP2K5-S142 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-S142 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | DB |
| DB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MEK5, MKK5, PRKMK5 |
|---|---|
| Alternative Names | MAP2K5 MEK5 MKK5 PRKMK5 Dual specificity mitogen-activated protein kinase kinase 5 MAPK/ERK kinase 5 |
| Molecular Weight(MW) | 50112 Da |
| Function | Acts as a scaffold for the formation of a ternary MAP3K2/MAP3K3-MAP3K5-MAPK7 signaling complex. Activation of this pathway appears to play a critical role in protecting cells from stress-induced apoptosis, neuronal survival and cardiac development and angiogenesis |
| Tissue Specificity | Expressed in many adult tissues. Abundant in heart and skeletal muscle |
| Entrez Gene | 5607 |
|---|
Application
Dot blot analysis of Phospho-MAP2K5-S142 polyclonal antibody (Cat# AP3343a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentration was 0.5ug per ml. P-Pab: phospho-antibody; P-Peptide: phospho-peptide; NP-Peptide: non-phospho-peptide.| Application Notes | DB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification. |
