Application
Dot blot analysis of anti-Phospho-PDX1-pS66 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.| Product Name | Phospho-PDX1-pS66 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PDHX; PDX1; Pyruvate dehydrogenase protein X component, mitochondrial; Dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenase complex; E3-binding protein; Lipoyl-containing pyruvate dehydrogenase complex component X; proX |
| Modification | p-S66 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | DB |
| DB:1:500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PDX1 |
|---|---|
| Alternative Names | PDHX PDX1 Pyruvate dehydrogenase protein X component mitochondrial Dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenase complex E3-binding protein Lipoyl-containing pyruvate dehydrogenase complex component X proX |
| Molecular Weight(MW) | 54122 Da |
| Function | Required for anchoring dihydrolipoamide dehydrogenase (E3) to the dihydrolipoamide transacetylase (E2) core of the pyruvate dehydrogenase complexes of eukaryotes. This specific binding is essential for a functional PDH complex |
| Tissue Specificity | This Phospho-PDX1-pS66 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between 55~84 amino acids surrounding S66 of human PDX1. |
| Cellular Localization | Mitochondrion matrix. |
| Entrez Gene | 8050 |
|---|
Application
Dot blot analysis of anti-Phospho-PDX1-pS66 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.| Application Notes | DB:1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification. |
