Application
Dot blot analysis of anti-PTPa Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Product Name | Phospho-PTPa-S180 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PTPRA; PTPA; PTPRL2; Receptor-type tyrosine-protein phosphatase alpha |
| Modification | p-S180 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | DBIHC |
| DB:1:500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PTPA PTPRL2 |
|---|---|
| Alternative Names | PTPRA PTPA PTPRL2 Receptor-type tyrosine-protein phosphatase alpha |
| Molecular Weight(MW) | 90600 Da |
| Tissue Specificity | This Phospho-PTPa-S180 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between 166~196 amino acids surrounding S180 of human PTPa. |
| Cellular Localization | Membrane; Single-pass type I membrane protein |
| Entrez Gene | 5786 |
|---|
Application
Dot blot analysis of anti-PTPa Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Application Notes | DB:1:500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
