Application
Western blot analysis of PHPT1-Y93 in MCF-7 cell line lysates (35ug/lane).PHPT1 (arrow) was detected using the purified Pab.Application
Western blot analysis of PHPT1-Y93 (arrow) using PHPT1 Antibody (Y93) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PHPT1 gene (Lane 2) (Origene Technologies).Application
Western blot analysis of PHPT1-Y93 in mouse NIH-3T3 cell line lysates (35ug/lane). PHPT1 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with Phospho-PHPT1-Y93.ctrl, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | PHPT1 Antibody (Y93) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PHPT1; PHP14; 14 kDa phosphohistidine phosphatase; Phosphohistidine phosphatase 1; Protein janus-A homolog |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~200 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PHP14 |
|---|---|
| Alternative Names | PHPT1 PHP14 14 kDa phosphohistidine phosphatase Phosphohistidine phosphatase 1 Protein janus-A homolog |
| Molecular Weight(MW) | 13833 Da |
| Function | Exhibits phosphohistidine phosphatase activity. |
| Tissue Specificity | Expressed abundantly in heart and skeletal muscle |
| Cellular Localization | Cytoplasm (By similarity). |
| Entrez Gene | 29085 |
|---|---|
| Nucleotide Accession | NP_054891.2 |
Application
Western blot analysis of PHPT1-Y93 in MCF-7 cell line lysates (35ug/lane).PHPT1 (arrow) was detected using the purified Pab.
Application
Western blot analysis of PHPT1-Y93 (arrow) using PHPT1 Antibody (Y93) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PHPT1 gene (Lane 2) (Origene Technologies).
Application
Western blot analysis of PHPT1-Y93 in mouse NIH-3T3 cell line lysates (35ug/lane). PHPT1 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with Phospho-PHPT1-Y93.ctrl, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:50~200 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
