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PI3KCB Antibody (C-term)
Catalog Number:OM217851
OM217851
Discount Price
OM217851-100ul
1~2 Week
100ul
OM217851-50ul
50ul
OM217851-20ul
20ul
1~2 Week
Product Profile
Product Name PI3KCB Antibody (C-term)
Antibody Type Primary Antibodies
Antigen Alias PIK3CB; PIK3C1; Phosphatidylinositol 4, 5-bisphosphate 3-kinase catalytic subunit beta isoform; Phosphatidylinositol 4, 5-bisphosphate 3-kinase 110 kDa catalytic subunit beta
Key Feature
Clonality Polyclonal
Isotype Ig
Host Species Rabbit
Tested Applications WBIHC
WB:1:100~500 IHC
Species Reactivity Human
Concentration 1mg/ml
Target Information
Gene Synonyms PIK3C1
Alternative Names PIK3CB
PIK3C1
Phosphatidylinositol 4
5-bisphosphate 3-kinase catalytic subunit beta isoform
Phosphatidylinositol 4
5-bisphosphate 3-kinase 110 kDa catalytic subunit beta
Molecular Weight(MW) 122762 Da
Function Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4- phosphate) and PtdIns(4,5)P2 (Phosphatidylinositol 4,5- bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Involved in the activation of AKT1 upon stimulation by G-protein coupled receptors (GPCRs) ligands such as CXCL12, sphingosine 1-phosphate, and lysophosphatidic acid. May also act downstream receptor tyrosine kinases. Required in different signaling pathways for stable platelet adhesion and aggregation. Plays a role in platelet activation signaling triggered by GPCRs, alpha-IIb/beta-3 integrins (ITGA2B/ ITGB3) and ITAM (immunoreceptor tyrosine-based activation motif)-bearing receptors such as GP6. Regulates the strength of adhesion of ITGA2B/ ITGB3 activated receptors necessary for the cellular transmission of contractile forces Required for platelet aggregation induced by F2 (thrombin) and thromboxane A2 (TXA2). Has a role in cell survival. May have a role in cell migration. Involved in the early stage of autophagosome formation. Modulates the intracellular level of PtdIns3P (Phosphatidylinositol 3-phosphate) and activates PIK3C3 kinase activity. May act as a scaffold, independently of its lipid kinase activity to positively regulate autophagy. May have a role in insulin signaling as scaffolding protein in which the lipid kinase activity is not required. May have a kinase-independent function in regulating cell proliferation and in clathrin-mediated endocytosis. Mediator of oncogenic signal in cell lines lacking PTEN. The lipid kinase activity is necessary for its role in oncogenic transformation. Required for the growth of ERBB2 and RAS driven tumors
Tissue Specificity Expressed ubiquitously.
Cellular Localization Cytoplasm. Nucleus. Note=Interaction with PIK3R2 is required for nuclear localization and export
Database Links
Entrez Gene 5291
Application

Application

PI3KCB Antibody (C-term K727) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the PI3KCB antibody detected the PI3KCB protein (arrow).

Application

Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with PI3KCB antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application Notes WB:1:100~500 IHC
Additional Information
Form Liquid
Storage Instructions For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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