Application
A. Western blot analysis on Jurkat cell lysate using anti-PI3-kinase p110 subunit beta (N-term) RabMAb (Cat. #AJ1607b), dilution 1:500.| Product Name | PI3-kinase (p110) Antibody (subunit beta) (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PIK3CB, PIK3C1, Phosphatidylinositol-4, 5-bisphosphate 3-kinase catalytic subunit beta isoform, PI3-kinase p110 subunit beta;PtdIns-3-kinase p110;PI3Kbeta |
| Clonality | Monoclonal |
|---|---|
| Clone Number | Y384 |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PIK3C1 |
|---|---|
| Alternative Names | PIK3CB PIK3C1 Phosphatidylinositol-4 5-bisphosphate 3-kinase catalytic subunit beta isoform PI3-kinase p110 subunit beta PtdIns-3-kinase p110 PI3Kbeta |
| Molecular Weight(MW) | 122762 Da |
| Function | Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4- phosphate) and PtdIns(4,5)P2 (Phosphatidylinositol 4,5- bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Involved in the activation of AKT1 upon stimulation by G-protein coupled receptors (GPCRs) ligands such as CXCL12, sphingosine 1-phosphate, and lysophosphatidic acid. May also act downstream receptor tyrosine kinases. Required in different signaling pathways for stable platelet adhesion and aggregation. Plays a role in platelet activation signaling triggered by GPCRs, alpha-IIb/beta-3 integrins (ITGA2B/ ITGB3) and ITAM (immunoreceptor tyrosine-based activation motif)-bearing receptors such as GP6. Regulates the strength of adhesion of ITGA2B/ ITGB3 activated receptors necessary for the cellular transmission of contractile forces Required for platelet aggregation induced by F2 (thrombin) and thromboxane A2 (TXA2). Has a role in cell survival. May have a role in cell migration. Involved in the early stage of autophagosome formation. Modulates the intracellular level of PtdIns3P (Phosphatidylinositol 3-phosphate) and activates PIK3C3 kinase activity. May act as a scaffold, independently of its lipid kinase activity to positively regulate autophagy. May have a role in insulin signaling as scaffolding protein in which the lipid kinase activity is not required. May have a kinase-independent function in regulating cell proliferation and in clathrin-mediated endocytosis. Mediator of oncogenic signal in cell lines lacking PTEN. The lipid kinase activity is necessary for its role in oncogenic transformation. Required for the growth of ERBB2 and RAS driven tumors |
| Tissue Specificity | Expressed ubiquitously. |
| Cellular Localization | Cytoplasm. Nucleus. Note=Interaction with PIK3R2 is required for nuclear localization and export |
| Entrez Gene | 5291 |
|---|
Application
A. Western blot analysis on Jurkat cell lysate using anti-PI3-kinase p110 subunit beta (N-term) RabMAb (Cat. #AJ1607b), dilution 1:500.| Application Notes | WB:1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
Data sheet for OM217832