Application
Untreated (–) and treated (+) HeLa whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody [GT664] diluted at 1:1000.| Product Name | Heme Oxygenase 1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [GT664] to Heme Oxygenase 1 (heme oxygenase (decycling) 1)Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Heme Oxygenase 1. The exact sequence is proprietary. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | GT664 |
| Host Species | Mouse |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Pig, BovineMouse (81%), Rat (80%), Dog (85%), Pig (82%), Bovine (83%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HMOX1 antibody HMOX1D antibody HO-1 antibody HO1 antibody HSP32 antibody Heme Oxygenase 1 antibody bK286B10 antibody |
|---|---|
| Target | Heme Oxygenase 1 |
| Molecular Weight(MW) | 33 kDa (note) |
| Cellular Localization | Microsome , Endoplasmic reticulum |
Application
Untreated (–) and treated (+) HeLa whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody [GT664] diluted at 1:1000.| Positive Control | HeLa , HeLa 500 μM CoCl2 treatment for 24 hr |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
