Application
Western blot analysis of PIST Antibody (Center) in A375 cell line lysates (35ug/lane). PIST (arrow) was detected using the purified Pab (1:60 dilution).Application
Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with PIST antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | PIST Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GOPC; CAL; FIG; Golgi-associated PDZ and coiled-coil motif-containing protein; CFTR-associated ligand; Fused in glioblastoma; PDZ protein interacting specifically with TC10 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CAL FIG |
|---|---|
| Alternative Names | GOPC CAL FIG Golgi-associated PDZ and coiled-coil motif-containing protein CFTR-associated ligand Fused in glioblastoma PDZ protein interacting specifically with TC10 |
| Molecular Weight(MW) | 50520 Da |
| Function | Plays a role in intracellular protein trafficking and degradation. May regulate CFTR chloride currents and acid-induced ASIC3 currents by modulating cell surface expression of both channels. May also regulate the intracellular trafficking of the ADR1B receptor. May play a role in autophagy. Overexpression results in CFTR intracellular retention and degradation in the lysosomes |
| Tissue Specificity | Ubiquitously expressed. |
| Cellular Localization | Cytoplasm. Golgi apparatus membrane; Peripheral membrane protein. Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein. Cell junction, synapse (By similarity). Cell junction, synapse, postsynaptic cell membrane, postsynaptic density (By similarity). Cell projection, dendrite (By similarity). Note=Enriched in synaptosomal and postsynaptic densities (PSD) fractions (By similarity). Expressed in cell bodies and dendrites of Purkinje cells (By similarity). Localized at the trans-Golgi network (TGN) of spermatids and the medulla of round spermatides (By similarity) |
| Entrez Gene | 57120 |
|---|
Application
Western blot analysis of PIST Antibody (Center) in A375 cell line lysates (35ug/lane). PIST (arrow) was detected using the purified Pab (1:60 dilution).
Application
Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with PIST antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified rabbit polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
