Application
Western blot analysis of PITX3 Antibody (N-term) in 293 cell line lysates (35ug/lane). PITX3 (arrow) was detected using the purified Pab.| Product Name | PITX3 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PITX3; PTX3; Pituitary homeobox 3; Homeobox protein PITX3; Paired-like homeodomain transcription factor 3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PTX3 |
|---|---|
| Alternative Names | PITX3 PTX3 Pituitary homeobox 3 Homeobox protein PITX3 Paired-like homeodomain transcription factor 3 |
| Molecular Weight(MW) | 31832 Da |
| Function | Transcriptional regulator which is important for the differentiation and maintenance of meso-diencephalic dopaminergic (mdDA) neurons during development. In addition to its importance during development, it also has roles in the long-term survival and maintenance of the mdDA neurons. Activates NR4A2/NURR1- mediated transcription of genes such as SLC6A3, SLC18A2, TH and DRD2 which are essential for development of mdDA neurons. Acts by decreasing the interaction of NR4A2/NURR1 with the corepressor NCOR2/SMRT which acts through histone deacetylases (HDACs) to keep promoters of NR4A2/NURR1 target genes in a repressed deacetylated state. Essential for the normal lens development and differentiation. Plays a critical role in the maintenance of mitotic activity of lens epithelial cells, fiber cell differentiation and in the control of the temporal and spatial activation of fiber cell-specific crystallins. Positively regulates FOXE3 expression and negatively regulates PROX1 in the anterior lens epithelium, preventing activation of CDKN1B/P27Kip1 and CDKN1C/P57Kip2 and thus maintains lens epithelial cells in cell cycle (By similarity) |
| Tissue Specificity | Highly expressed in developing eye lens. |
| Cellular Localization | Nucleus (By similarity). |
| Entrez Gene | 5309 |
|---|
Application
Western blot analysis of PITX3 Antibody (N-term) in 293 cell line lysates (35ug/lane). PITX3 (arrow) was detected using the purified Pab.| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
