Application
Western blot analysis of PITX1 antibody (Center) in NCI-H460 cell line lysates (35ug/lane). PITX1 (arrow) was detected using the purified Pab.Application
PITX1 Antibody (Center) (RB18913) IHC analysis in formalin fixed and paraffin embedded human Lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PITX1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
PITX1 Antibody (Center) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of PITX1 (arrow) using rabbit polyclonal PITX1 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PITX1 gene.| Product Name | PITX1 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PITX1; BFT; PTX1; Pituitary homeobox 1; Hindlimb-expressed homeobox protein backfoot; Homeobox protein PITX1; Paired-like homeodomain transcription factor 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | BFT PTX1 |
|---|---|
| Alternative Names | PITX1 BFT PTX1 Pituitary homeobox 1 Hindlimb-expressed homeobox protein backfoot Homeobox protein PITX1 Paired-like homeodomain transcription factor 1 |
| Molecular Weight(MW) | 34128 Da |
| Function | May play a role in the development of anterior structures, and in particular, the brain and facies and in specifying the identity or structure of hindlimb |
| Tissue Specificity | This PITX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 129~159 amino acids from the Center region of human PITX1. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 5307 |
|---|
Application
Western blot analysis of PITX1 antibody (Center) in NCI-H460 cell line lysates (35ug/lane). PITX1 (arrow) was detected using the purified Pab.
Application
PITX1 Antibody (Center) (RB18913) IHC analysis in formalin fixed and paraffin embedded human Lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PITX1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
PITX1 Antibody (Center) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of PITX1 (arrow) using rabbit polyclonal PITX1 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PITX1 gene.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
