Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.Application
The anti-PKLR Pab is used in Western blot to detect PKLR in mouse liver tissue lysate.Application
PKLR Antibody (G536) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the PKLR antibody detected the PKLR protein (arrow).| Product Name | PKLR Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PKLR; PK1; PKL; Pyruvate kinase isozymes R/L; Pyruvate kinase 1; R-type/L-type pyruvate kinase; Red cell/liver pyruvate kinase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| IHC:1:50~100 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PK1 PKL |
|---|---|
| Alternative Names | PKLR PK1 PKL Pyruvate kinase isozymes R/L Pyruvate kinase 1 R-type/L-type pyruvate kinase Red cell/liver pyruvate kinase |
| Molecular Weight(MW) | 61830 Da |
| Function | Plays a key role in glycolysis (By similarity). |
| Tissue Specificity | This PKLR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 529~558 amino acids from the C-terminal region of human PKLR. |
| Entrez Gene | 5313 |
|---|
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Application
The anti-PKLR Pab is used in Western blot to detect PKLR in mouse liver tissue lysate.
Application
PKLR Antibody (G536) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the PKLR antibody detected the PKLR protein (arrow).| Application Notes | IHC:1:50~100 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
