Application
Western blot analysis of PKM1 antibody (C-term L398) in Hela cell line lysates (35ug/lane). PKM1 (arrow) was detected using the purified Pab.Application
Western blot analysis of PKM1 antibody (C-term L398) in mouse testis tissue lysates (35ug/lane). PKM1 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with PKM1 Antibody (C-term L398), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | PKM1 Antibody (C-term L398) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Pyruvate kinase isozymes M1/M2; Pyruvate kinase muscle isozyme; Pyruvate kinase 2/3; Cytosolic thyroid hormone-binding protein; CTHBP; THBP1 ; PKM; PK3; PK2;OIP3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | OIP3 PK2 PK3 PKM2 |
|---|---|
| Alternative Names | Pyruvate kinase isozymes M1/M2 Pyruvate kinase muscle isozyme Pyruvate kinase 2/3 Cytosolic thyroid hormone-binding protein CTHBP THBP1 PKM PK3 PK2 OIP3 |
| Molecular Weight(MW) | 57937 Da |
| Function | Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival |
| Tissue Specificity | Specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells |
| Cellular Localization | Cytoplasm. Nucleus. Note=Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic activity |
Application
Western blot analysis of PKM1 antibody (C-term L398) in Hela cell line lysates (35ug/lane). PKM1 (arrow) was detected using the purified Pab.
Application
Western blot analysis of PKM1 antibody (C-term L398) in mouse testis tissue lysates (35ug/lane). PKM1 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with PKM1 Antibody (C-term L398), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
