Application
Western blot analysis of PLA2G4A Antibody (Center) in NCI-H460 cell line lysates (35ug/lane). PLA2G4A (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human lung carcinoma with PLA2G4A Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of PLA2G4A Antibody (Center) with NCI-H460 cell followed by Alexa Fluor搴?488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
Flow cytometric analysis of NCI-H292 cells using PLA2G4A Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | PLA2G4A Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PLA2G4A; CPLA2; PLA2G4; Cytosolic phospholipase A2; Phospholipase A2 group IVA; Phospholipase A2; Phosphatidylcholine 2-acylhydrolase; Lysophospholipase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIFFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CPLA2 PLA2G4 |
|---|---|
| Alternative Names | PLA2G4A CPLA2 PLA2G4 Cytosolic phospholipase A2 Phospholipase A2 group IVA Phospholipase A2 Phosphatidylcholine 2-acylhydrolase Lysophospholipase |
| Molecular Weight(MW) | 85239 Da |
| Function | Selectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Together with its lysophospholipid activity, it is implicated in the initiation of the inflammatory response |
| Tissue Specificity | Expressed in various tissues such as macrophages, platelets, neutrophils, fibroblasts and lung endothelium |
| Cellular Localization | Cytoplasm. Cytoplasmic vesicle. Note=Translocates to membrane vesicles in a calcium-dependent fashion |
| Entrez Gene | 5321 |
|---|
Application
Western blot analysis of PLA2G4A Antibody (Center) in NCI-H460 cell line lysates (35ug/lane). PLA2G4A (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human lung carcinoma with PLA2G4A Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of PLA2G4A Antibody (Center) with NCI-H460 cell followed by Alexa Fluor搴?488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
Flow cytometric analysis of NCI-H292 cells using PLA2G4A Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
