Application
Western blot analysis of Phospho-PLB-pS16 in A375 cell line lysates (36ug/lane). PLB (arrow) was detected using the purified Pab.Application
PLB-S16 Antibody IHC analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PLB-S16 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.Application
PLB-S16 Antibody flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | PLB-S16 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PLN; PLB; Cardiac phospholamban |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PLB |
|---|---|
| Alternative Names | PLN PLB Cardiac phospholamban |
| Molecular Weight(MW) | 6109 Da |
| Function | Phospholamban has been postulated to regulate the activity of the calcium pump of cardiac sarcoplasmic reticulum |
| Tissue Specificity | Heart. |
| Cellular Localization | Mitochondrion membrane; Single-pass membrane protein. Sarcoplasmic reticulum (By similarity) |
| Entrez Gene | 5350 |
|---|
Application
Western blot analysis of Phospho-PLB-pS16 in A375 cell line lysates (36ug/lane). PLB (arrow) was detected using the purified Pab.
Application
PLB-S16 Antibody IHC analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PLB-S16 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
Application
PLB-S16 Antibody flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
