Application
A. Western blot analysis on Jurkat cell lysates using anti-Phospho- PLC gamma 1 (pY783) RabMAb (Cat. #AJ1631c), 1:10,000 dilution. Cells were either (A) untreated (B) treated with pervanadate.| Product Name | PLCI Antibody Phospho (pY783) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PLCG1, PLC1, 1-phosphatidylinositol-4, 5-bisphosphate phosphodiesterase gamma-1, PLC-II;PLC-148 |
| Modification | p-Y783 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR2611Y |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:50000~100000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PLC1 |
|---|---|
| Alternative Names | PLCG1 PLC1 1-phosphatidylinositol-4 5-bisphosphate phosphodiesterase gamma-1 PLC-II PLC-148 |
| Molecular Weight(MW) | 148532 Da |
| Function | Mediates the production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). Plays an important role in the regulation of intracellular signaling cascades. Becomes activated in response to ligand- mediated activation of receptor-type tyrosine kinases, such as PDGFRA, PDGFRB, FGFR1, FGFR2, FGFR3 and FGFR4. Plays a role in actin reorganization and cell migration |
| Tissue Specificity | A phospho specific peptide corresponding to residues surrounding tyrosine 783 of human PLCgamma1 was used as an immunogen. This antibody detects PLCgamma1 phosphorylated on tyrosine 783. |
| Cellular Localization | Cell projection, lamellipodium. Cell projection, ruffle. Note=Rapidly redistributed to ruffles and lamellipodia structures in response to epidermal growth factor (EGF) treatment |
| Entrez Gene | 5335 |
|---|
Application
A. Western blot analysis on Jurkat cell lysates using anti-Phospho- PLC gamma 1 (pY783) RabMAb (Cat. #AJ1631c), 1:10,000 dilution. Cells were either (A) untreated (B) treated with pervanadate.| Application Notes | WB:1:50000~100000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
