Application
Western blot analysis of anti-T1A-2 Pab in NCI-H460 cell lysate. T1A-2 (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.| Product Name | Podoplanin / GP36 / T1A-2 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PDPN; GP36; Podoplanin; PA2.26 antigen; T1-alpha; Aggrus; Glycoprotein 36 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | GP36 |
|---|---|
| Alternative Names | PDPN GP36 Podoplanin PA2.26 antigen T1-alpha Aggrus Glycoprotein 36 |
| Molecular Weight(MW) | 16698 Da |
| Function | May be involved in cell migration and/or actin cytoskeleton organization. When expressed in keratinocytes, induces changes in cell morphology with transfected cells showing an elongated shape, numerous membrane protrusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion. Required for normal lung cell proliferation and alveolus formation at birth. Induces platelet aggregation. Does not have any effect on folic acid or amino acid transport. Does not function as a water channel or as a regulator of aquaporin-type water channels |
| Tissue Specificity | Highly expressed in placenta, lung, skeletal muscle and brain. Weakly expressed in brain, kidney and liver. In placenta, expressed on the apical plasma membrane of endothelium In lung, expressed in alveolar epithelium. Up-regulated in colorectal tumors and expressed in 25% of early oral squamous cell carcinomas |
| Cellular Localization | Membrane; Single-pass type I membrane protein (By similarity). Cell projection, filopodium membrane; Single-pass type I membrane protein (By similarity). Cell projection, lamellipodium membrane; Single-pass type I membrane protein (By similarity). Cell projection, microvillus membrane; Single-pass type I membrane protein (By similarity). Cell projection, ruffle membrane; Single-pass type I membrane protein (By similarity). Note=Localized to actin-rich microvilli and plasma membrane projections such as filopodia, lamellipodia and ruffles (By similarity) |
Application
Western blot analysis of anti-T1A-2 Pab in NCI-H460 cell lysate. T1A-2 (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
