Application
Western blot analysis of PRDX6 Antibody (C-term) in CEM cell line lysates (35ug/lane). PRDX6 (arrow) was detected using the purified Pab.Application
Western blot analysis of PRDX6 (arrow) using rabbit polyclonal PRDX6 Antibody (C-term) (RB20894). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRDX6 gene (Lane 2) .Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with PRDX6 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
PRDX6 Antibody (C-term) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | PRDX6 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PRDX6; AOP2; KIAA0106; Peroxiredoxin-6; 1-Cys peroxiredoxin; 24 kDa protein; Acidic calcium-independent phospholipase A2; Antioxidant protein 2; Liver 2D page spot 40; Non-selenium glutathione peroxidase; Red blood cells page spot 12 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | AOP2 KIAA0106 |
|---|---|
| Alternative Names | PRDX6 AOP2 KIAA0106 Peroxiredoxin-6 1-Cys peroxiredoxin 24 kDa protein Acidic calcium-independent phospholipase A2 Antioxidant protein 2 Liver 2D page spot 40 Non-selenium glutathione peroxidase Red blood cells page spot 12 |
| Molecular Weight(MW) | 25035 Da |
| Function | Involved in redox regulation of the cell. Can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. May play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury |
| Tissue Specificity | This PRDX6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 195~224 amino acids from the C-terminal region of human PRDX6. |
| Cellular Localization | Cytoplasm. Lysosome (By similarity). Cytoplasmic vesicle (By similarity). Note=Also found in lung secretory organelles (By similarity) |
| Entrez Gene | 9588 |
|---|
Application
Western blot analysis of PRDX6 Antibody (C-term) in CEM cell line lysates (35ug/lane). PRDX6 (arrow) was detected using the purified Pab.
Application
Western blot analysis of PRDX6 (arrow) using rabbit polyclonal PRDX6 Antibody (C-term) (RB20894). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRDX6 gene (Lane 2) .
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with PRDX6 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
PRDX6 Antibody (C-term) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
