Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.Application
The anti-PRMT2 Pab is used in Western blot to detect PRMT2 in HL60 cell lysate.Application
Western blot analysis of PRMT2 (arrow) using rabbit polyclonal PRMT2 Antibody (A37) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PRMT2 gene.| Product Name | PRMT2 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PRMT2; HMT1; HRMT1L1; Protein arginine N-methyltransferase 2; Histone-arginine N-methyltransferase PRMT2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| IHC:1:50~100 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HMT1 HRMT1L1 |
|---|---|
| Alternative Names | PRMT2 HMT1 HRMT1L1 Protein arginine N-methyltransferase 2 Histone-arginine N-methyltransferase PRMT2 |
| Molecular Weight(MW) | 49042 Da |
| Function | Arginine methyltransferase that methylates the guanidino nitrogens of arginyl residues in proteins such as STAT3, FBL, histone H4. Acts as a coactivator (with NCOA2) of the androgen receptor (AR)-mediated transactivation. Acts as a coactivator (with estrogen) of estrogen receptor (ER)-mediated transactivation. Enhances PGR, PPARG, RARA-mediated transactivation. May inhibit NF-kappa-B transcription and promote apoptosis. Represses E2F1 transcriptional activity (in a RB1- dependent manner). May be involved in growth regulation |
| Tissue Specificity | Widely expressed. Highly expressed in androgen target organs such as heart, prostate, skeletal muscle, ovary and spinal cord |
| Cellular Localization | Cytoplasm. Nucleus. Note=Translocates from the cytoplasm to the nucleus, after hormone exposure |
| Entrez Gene | 3275 |
|---|
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Application
The anti-PRMT2 Pab is used in Western blot to detect PRMT2 in HL60 cell lysate.
Application
Western blot analysis of PRMT2 (arrow) using rabbit polyclonal PRMT2 Antibody (A37) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PRMT2 gene.| Application Notes | IHC:1:50~100 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
