Application
Western blot analysis of anti-PRMT3 Pab in whole HL60 cell lysate: PRMT3 (Arrow) was detected using purified Pab (Lane B) but not pre-immune serum (lane A). Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.Application
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with PRMT3 Antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | PRMT3 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PRMT3; HRMT1L3; Protein arginine N-methyltransferase 3; Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HRMT1L3 |
|---|---|
| Alternative Names | PRMT3 HRMT1L3 Protein arginine N-methyltransferase 3 Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 3 |
| Molecular Weight(MW) | 59876 Da |
| Function | Methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in some proteins |
| Tissue Specificity | This PRMT3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 449~479 amino acids from the C-terminal region of human PRMT3. |
| Cellular Localization | Cytoplasm. |
| Entrez Gene | 10196 |
|---|
Application
Western blot analysis of anti-PRMT3 Pab in whole HL60 cell lysate: PRMT3 (Arrow) was detected using purified Pab (Lane B) but not pre-immune serum (lane A). Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Application
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with PRMT3 Antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
