Application
Western blot analysis of PSA Antibody (Center) in MCF-7 and Hela cell line lysates (35ug/lane). PSA (arrow) was detected using the purified Pab.Application
PSA Antibody (Center) IHC analysis in formalin fixed and paraffin embedded human Lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PSA Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
PSA Antibody (Center) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | PSA Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NPEPPS; PSA; Puromycin-sensitive aminopeptidase; Cytosol alanyl aminopeptidase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PSA |
|---|---|
| Alternative Names | NPEPPS PSA Puromycin-sensitive aminopeptidase Cytosol alanyl aminopeptidase |
| Molecular Weight(MW) | 103276 Da |
| Function | Aminopeptidase with broad substrate specificity for several peptides. Involved in proteolytic events essential for cell growth and viability. May act as regulator of neuropeptide activity. Plays a role in the antigen-processing pathway for MHC class I molecules. Involved in the N-terminal trimming of cytotoxic T-cell epitope precursors. Digests the poly-Q peptides found in many cellular proteins. Digests tau from normal brain more efficiently than tau from Alzheimer disease brain |
| Tissue Specificity | Detected in liver, epithelium of renal tubules, epithelium of small and large intestine, gastric epithelial cells, and alveoli of the lung (at protein level) |
| Cellular Localization | Cytoplasm, cytosol. Nucleus (Potential). |
| Entrez Gene | 9520 |
|---|
Application
Western blot analysis of PSA Antibody (Center) in MCF-7 and Hela cell line lysates (35ug/lane). PSA (arrow) was detected using the purified Pab.
Application
PSA Antibody (Center) IHC analysis in formalin fixed and paraffin embedded human Lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PSA Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
PSA Antibody (Center) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
