Application
Western blot analysis of PSMA2 Antibody (C-term) in HL60,293,CEM cell line lysates (35ug/lane).PSMA2 (arrow) was detected using the purified Pab.(2ug/ml)Application
Western blot analysis of PSMA2 (arrow) using rabbit polyclonal PSMA2 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PSMA2 gene (Lane 2).Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with PSMA2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
PSMA2 Antibody (C-term) flow cytometry analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | PSMA2 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PSMA2; HC3; PSC3; Proteasome subunit alpha type-2; Macropain subunit C3; Multicatalytic endopeptidase complex subunit C3; Proteasome component C3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HC3 PSC3 |
|---|---|
| Alternative Names | PSMA2 HC3 PSC3 Proteasome subunit alpha type-2 Macropain subunit C3 Multicatalytic endopeptidase complex subunit C3 Proteasome component C3 |
| Molecular Weight(MW) | 25899 Da |
| Function | The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. PSMA2 may have a potential regulatory effect on another component(s) of the proteasome complex through tyrosine phosphorylation |
| Tissue Specificity | This PSMA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 205~234 amino acids from the C-terminal region of human PSMA2. |
| Cellular Localization | Cytoplasm. Nucleus. Cytoplasm, P-body (By similarity). Note=Co-localizes with TRIM5 in the cytoplasmic bodies (By similarity) |
| Entrez Gene | 5683 |
|---|
Application
Western blot analysis of PSMA2 Antibody (C-term) in HL60,293,CEM cell line lysates (35ug/lane).PSMA2 (arrow) was detected using the purified Pab.(2ug/ml)
Application
Western blot analysis of PSMA2 (arrow) using rabbit polyclonal PSMA2 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PSMA2 gene (Lane 2).
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with PSMA2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
PSMA2 Antibody (C-term) flow cytometry analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
