Application
PSMB5 Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the PSMB5 antibody detected the PSMB5 protein (arrow).Application
Western blot analysis of PSMB5 (arrow) using rabbit polyclonal PSMB5 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PSMB5 gene.Application
PSMB5 Antibody (C-term) (AP14528b)immunohistochemistry analysis in formalin fixed and paraffin embedded human skin tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PSMB5 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | PSMB5 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PSMB5; LMPX; MB1; X; Proteasome subunit beta type-5; Macropain epsilon chain; Multicatalytic endopeptidase complex epsilon chain; Proteasome chain 6; Proteasome epsilon chain; Proteasome subunit MB1; Proteasome subunit X |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | LMPX MB1 X |
|---|---|
| Alternative Names | PSMB5 LMPX MB1 X Proteasome subunit beta type-5 Macropain epsilon chain Multicatalytic endopeptidase complex epsilon chain Proteasome chain 6 Proteasome epsilon chain Proteasome subunit MB1 Proteasome subunit X |
| Molecular Weight(MW) | 28480 Da |
| Function | The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This unit is responsible of the chymotrypsin-like activity of the proteasome and is one of the principal target of the proteasome inhibitor bortezomib. May catalyze basal processing of intracellular antigens. Plays a role in the protection against oxidative damage through the Nrf2-ARE pathway (By similarity) |
| Tissue Specificity | This PSMB5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 234-263 amino acids from the C-terminal region of human PSMB5. |
| Cellular Localization | Cytoplasm. Nucleus. |
| Entrez Gene | 5693 |
|---|---|
| Nucleotide Accession | NP_001124197.1 NP_002788.1 |
Application
PSMB5 Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the PSMB5 antibody detected the PSMB5 protein (arrow).
Application
Western blot analysis of PSMB5 (arrow) using rabbit polyclonal PSMB5 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PSMB5 gene.
Application
PSMB5 Antibody (C-term) (AP14528b)immunohistochemistry analysis in formalin fixed and paraffin embedded human skin tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PSMB5 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
