Application
A. Western blot analysis on Hela cell lysates using anti-Phospho-PTP1B (pS50) RabMAb (Cat. #AJ1665e), 1:5000 dilution. Cells were either (A) untreated (B) treated with Calyculin A.| Product Name | PTP1B Antibody Phospho (pS50) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-S50 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP1838Y |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000~10000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PTP1B |
|---|---|
| Alternative Names | PTPN1 PTP1B Tyrosine-protein phosphatase non-receptor type 1 Protein-tyrosine phosphatase 1B |
| Molecular Weight(MW) | 49967 Da |
| Function | Tyrosine-protein phosphatase which acts as a regulator of endoplasmic reticulum unfolded protein response. Mediates dephosphorylation of EIF2AK3/PERK; inactivating the protein kinase activity of EIF2AK3/PERK. May play an important role in CKII- and p60c-src-induced signal transduction cascades. May regulate the EFNA5-EPHA3 signaling pathway which modulates cell reorganization and cell-cell repulsion |
| Tissue Specificity | A phospho specific peptide corresponding to residues surround Serine 50 of human PTP1B was used as an immunogen. This antibody detects PTP1B phosphorylated at Serine 50. |
| Cellular Localization | Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Note=Interacts with EPHA3 at the cell membrane |
| Entrez Gene | 5770 |
|---|
Application
A. Western blot analysis on Hela cell lysates using anti-Phospho-PTP1B (pS50) RabMAb (Cat. #AJ1665e), 1:5000 dilution. Cells were either (A) untreated (B) treated with Calyculin A.| Application Notes | WB:1:1000~10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
