Application
Western blot analysis of anti-PTP1B Ctr in HepG2 cell line lysate (35ug/lane).PTP1B Ctr(arrow) was detected using the purified Pab (1:40 dilution).Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PTP1B antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | PTP1B Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PTP1B |
|---|---|
| Alternative Names | PTPN1 PTP1B Tyrosine-protein phosphatase non-receptor type 1 Protein-tyrosine phosphatase 1B |
| Molecular Weight(MW) | 49967 Da |
| Function | Tyrosine-protein phosphatase which acts as a regulator of endoplasmic reticulum unfolded protein response. Mediates dephosphorylation of EIF2AK3/PERK; inactivating the protein kinase activity of EIF2AK3/PERK. May play an important role in CKII- and p60c-src-induced signal transduction cascades. May regulate the EFNA5-EPHA3 signaling pathway which modulates cell reorganization and cell-cell repulsion |
| Tissue Specificity | This PTP1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human PTP1B. |
| Cellular Localization | Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Note=Interacts with EPHA3 at the cell membrane |
| Entrez Gene | 5770 |
|---|
Application
Western blot analysis of anti-PTP1B Ctr in HepG2 cell line lysate (35ug/lane).PTP1B Ctr(arrow) was detected using the purified Pab (1:40 dilution).
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PTP1B antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
