Application
Western blot analysis of PUM1 (arrow) using PUM1 Antibody (Y83) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PUM1 gene (Lane 2) (Origene Technologies).Application
Formalin-fixed and paraffin-embedded humanbrain tissue reacted with Phospho-PUM1-Y83.ctrl antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | PUM1 Antibody (Y83) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA0099, PUMH1 |
|---|---|
| Alternative Names | PUM1 KIAA0099 PUMH1 Pumilio homolog 1 |
| Molecular Weight(MW) | 126473 Da |
| Function | Sequence-specific RNA-binding protein that regulates translation and mRNA stability by binding the 3'-UTR of mRNA targets. May be required to support proliferation and self-renewal of stem cells (By similarity) |
| Tissue Specificity | Expressed in brain, heart, kidney, muscle, intestine and stomach. Not expressed in cerebellum, corpus callosum, caudate nucleus, hippocampus, medulla oblongata and putamen. Expressed in all fetal tissues tested |
| Cellular Localization | Cytoplasm (Probable). |
| Entrez Gene | 9698 |
|---|
Application
Western blot analysis of PUM1 (arrow) using PUM1 Antibody (Y83) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PUM1 gene (Lane 2) (Origene Technologies).
Application
Formalin-fixed and paraffin-embedded humanbrain tissue reacted with Phospho-PUM1-Y83.ctrl antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
