Application
RALA Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the RALA antibody detected the RALA protein (arrow).| Product Name | RALA Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RALA; RAL; Ras-related protein Ral-A |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | RAL |
|---|---|
| Alternative Names | RALA RAL Ras-related protein Ral-A |
| Molecular Weight(MW) | 23567 Da |
| Function | Multifunctional GTPase involved in a variety of cellular processes including gene expression, cell migration, cell proliferation, oncogenic transformation and membrane trafficking Accomplishes its multiple functions by interacting with distinct downstream effectors. Acts as a GTP sensor for GTP-dependent exocytosis of dense core vesicles. Plays a role in the early stages of cytokinesis and is required to tether the exocyst to the cytokinetic furrow. The RALA-exocyst complex regulates integrin- dependent membrane raft exocytosis and growth signaling. Key regulator of LPAR1 signaling and competes with ADRBK1 for binding to LPAR1 thus affecting the signaling properties of the receptor Required for anchorage-independent proliferation of transformed cells |
| Tissue Specificity | This RALA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 158-186 amino acids from the C-terminal region of human RALA. |
| Cellular Localization | Cell surface. Cell membrane; Lipid-anchor; Cytoplasmic side. Cleavage furrow. Midbody. Note=Prior to LPA treatment found predominantly at the cell surface and in the presence of LPA co-localizes with LPAR1 and LPAR2 in the endocytic vesicles. During early cytokinesis localizes at the cleavage furrow membrane. Colocalizes with EXOC2 at the early midbody ring and persists there till maturation of the midbody |
| Entrez Gene | 5898 |
|---|---|
| Nucleotide Accession | NP_005393.2 |
Application
RALA Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the RALA antibody detected the RALA protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
