Application
A. Western blot analysis on Jurkat cell lysate using anti-Rap1GAP (N-term) RabMAb (Cat. #AJ1678b), dilution 1:50,000.Application
B. Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-Rap1GAP (N-term) RabMAb (Cat. #AJ1678b).| Product Name | Rap1GAP Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RAP1GAP, KIAA0474, RAP1GA1, Rap1 GTPase-activating protein 1 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | Y134 |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:5000~10000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA0474 RAP1GA1 |
|---|---|
| Alternative Names | RAP1GAP KIAA0474 RAP1GA1 Rap1 GTPase-activating protein 1 |
| Molecular Weight(MW) | 73361 Da |
| Function | GTPase activator for the nuclear Ras-related regulatory protein RAP-1A (KREV-1), converting it to the putatively inactive GDP-bound state |
| Tissue Specificity | Significant expression seen in the brain, kidney and pancreas. Abundant in the cerebral cortex and expressed at much lower levels in the spinal cord. Not detected in the lymphoid tissues |
| Cellular Localization | Golgi apparatus membrane; Peripheral membrane protein |
| Entrez Gene | 5909 |
|---|
Application
A. Western blot analysis on Jurkat cell lysate using anti-Rap1GAP (N-term) RabMAb (Cat. #AJ1678b), dilution 1:50,000.
Application
B. Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-Rap1GAP (N-term) RabMAb (Cat. #AJ1678b).| Application Notes | WB:1:5000~10000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
