Application
Western blot analysis of hRIPK3-E504 in K562 cell line lysates (35ug/lane). RIPK3 (arrow) was detected using the purified Pab.Application
Western blot analysis of hRIPK3-E504 in mouse cerebellum tissue lysates (35ug/lane). RIPK3 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with RIP3 (RIPK3) antibody (C-term) (Cat.# AP7819b), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | RIPK3 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RIPK3; RIP3; Receptor-interacting serine/threonine-protein kinase 3; RIP-like protein kinase 3; Receptor-interacting protein 3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | RIP3 |
|---|---|
| Alternative Names | RIPK3 RIP3 Receptor-interacting serine/threonine-protein kinase 3 RIP-like protein kinase 3 Receptor-interacting protein 3 |
| Molecular Weight(MW) | 56887 Da |
| Function | Essential for programmed necrosis in response to death- inducing TNF-alpha family members. Upon induction of necrosis, RIPK3 interacts with, and phosphorylates RIPK1 to form a necrosis- inducing complex. RIPK3 binds to and enhances the activity of three metabolic enzymes: GLUL, GLUD1, and PYGL. These metabolic enzymes may eventually stimulate the tricarboxylic acid cycle and oxidative phosphorylation, which could result in enhanced ROS production (By similarity) |
| Tissue Specificity | Highly expressed in the pancreas. Detected at lower levels in heart, placenta, lung and kidney. Isoform 3 is significantly increased in colon and lung cancers |
| Cellular Localization | Cytoplasm (By similarity). Cell membrane (By similarity). Mitochondrion (Potential) |
Application
Western blot analysis of hRIPK3-E504 in K562 cell line lysates (35ug/lane). RIPK3 (arrow) was detected using the purified Pab.
Application
Western blot analysis of hRIPK3-E504 in mouse cerebellum tissue lysates (35ug/lane). RIPK3 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with RIP3 (RIPK3) antibody (C-term) (Cat.# AP7819b), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
