Application
A. Western blot analysis on MCF7 cell lysate using anti-Rsk1 (N-term) RabMAb (Cat. #AJ1697b), dilution 1:1000.Application
B. IHC - Colon tissue section stained for RSK-1 subcellular location using anti-RSK-1.Application
C. Flow cytometric analysis of permeabilized Jurkat cells using anti-Rsk-1 RabMAb (red) (Cat. #AJ1697b) or a rabbit IgG (negative) (green).Application
D. Immunofluorescent staining of HeLa cells using anti-Rsk 1 RabMab (cat#1004-1).| Product Name | RSK1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RPS6KA1, RSK1, Ribosomal protein S6 kinase alpha-1, MAP kinase-activated protein kinase 1a |
| Clonality | Monoclonal |
|---|---|
| Clone Number | E4 |
| Host Species | Rabbit |
| Tested Applications | WBFCIF |
| WB:1:1000 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MAPKAPK1A RSK1 |
|---|---|
| Alternative Names | RPS6KA1 RSK1 Ribosomal protein S6 kinase alpha-1 MAP kinase-activated protein kinase 1a |
| Molecular Weight(MW) | 82723 Da |
| Function | Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77, regulates translation through RPS6 and EIF4B phosphorylation, and mediates cellular proliferation, survival, and differentiation by modulating mTOR signaling and repressing pro-apoptotic function of BAD and DAPK1. In fibroblast, is required for EGF-stimulated phosphorylation of CREB1, which results in the subsequent transcriptional activation of several immediate-early genes. In response to mitogenic stimulation (EGF and PMA), phosphorylates and activates NR4A1/NUR77 and ETV1/ER81 transcription factors and the cofactor CREBBP. Upon insulin- derived signal, acts indirectly on the transcription regulation of several genes by phosphorylating GSK3B at 'Ser-9' and inhibiting its activity. Phosphorylates RPS6 in response to serum or EGF via an mTOR-independent mechanism and promotes translation initiation by facilitating assembly of the preinitiation complex. In response to insulin, phosphorylates EIF4B, enhancing EIF4B affinity for the EIF3 complex and stimulating cap-dependent translation. Is involved in the mTOR nutrient-sensing pathway by directly phosphorylating TSC2 at 'Ser-1798', which potently inhibits TSC2 ability to suppress mTOR signaling, and mediates phosphorylation of RPTOR, which regulates mTORC1 activity and may promote rapamycin-sensitive signaling independently of the PI3K/AKT pathway. Mediates cell survival by phosphorylating the pro- apoptotic proteins BAD and DAPK1 and suppressing their pro- apoptotic function. Promotes the survival of hepatic stellate cells by phosphorylating CEBPB in response to the hepatotoxin carbon tetrachloride (CCl4). Is involved in cell cycle regulation by phosphorylating the CDK inhibitor CDKN1B, which promotes CDKN1B association with 14-3-3 proteins and prevents its translocation to the nucleus and inhibition of G1 progression |
| Tissue Specificity | A synthetic peptide corresponding to residues in N-terminus of human p90-RSK1 was used as immunogen. |
| Cellular Localization | Nucleus. Cytoplasm. |
| Entrez Gene | 6195 |
|---|
Application
A. Western blot analysis on MCF7 cell lysate using anti-Rsk1 (N-term) RabMAb (Cat. #AJ1697b), dilution 1:1000.
Application
B. IHC - Colon tissue section stained for RSK-1 subcellular location using anti-RSK-1.
Application
C. Flow cytometric analysis of permeabilized Jurkat cells using anti-Rsk-1 RabMAb (red) (Cat. #AJ1697b) or a rabbit IgG (negative) (green).
Application
D. Immunofluorescent staining of HeLa cells using anti-Rsk 1 RabMab (cat#1004-1).| Application Notes | WB:1:1000 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
