Application
Western blot analysis of RTCD1 Antibody (C-term) in CEM cell line lysates (35ug/lane). RTCD1 (arrow) was detected using the purified Pab.Application
Flow cytometric analysis of K562 cells using RTCD1 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of RTCD1 (arrow) using rabbit polyclonal RTCD1 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RTCD1 gene.| Product Name | RTCD1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RTCA; RPC; RPC1; RTC1; RTCD1; RNA 3'-terminal phosphate cyclase; RNA terminal phosphate cyclase domain-containing protein 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:50~100 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | RPC RPC1 RTC1 RTCD1 |
|---|---|
| Alternative Names | RTCA RPC RPC1 RTC1 RTCD1 RNA 3'-terminal phosphate cyclase RNA terminal phosphate cyclase domain-containing protein 1 |
| Molecular Weight(MW) | 39337 Da |
| Function | Catalyzes the conversion of 3'-phosphate to a 2',3'- cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA- N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'- phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing |
| Tissue Specificity | Ubiquitous. |
| Cellular Localization | Nucleus, nucleoplasm. |
| Entrez Gene | 8634 |
|---|
Application
Western blot analysis of RTCD1 Antibody (C-term) in CEM cell line lysates (35ug/lane). RTCD1 (arrow) was detected using the purified Pab.
Application
Flow cytometric analysis of K562 cells using RTCD1 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of RTCD1 (arrow) using rabbit polyclonal RTCD1 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RTCD1 gene.| Application Notes | WB:1:50~100 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
