Application
A. Western blot analysis on (A) Molt-4 and (B) fetal thymus lysates using anti-RUNX1 RabMAb (Cat. #AJ1701a), dilution 1:10,000.Application
B. Immunohistochemical analysis of paraffin-embedded human tonsils using anti-RUNX1 RabMAb (Cat. #AJ1701a).| Product Name | RUNX1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RUNX1, AML1, CBFA2, Runt-related transcription factor 1, SL3-3 enhancer factor 1 alpha B subunit;SL3/AKV core-binding factor alpha B subunit |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR3099 |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | AML1 CBFA2 |
|---|---|
| Alternative Names | RUNX1 AML1 CBFA2 Runt-related transcription factor 1 SL3-3 enhancer factor 1 alpha B subunit SL3/AKV core-binding factor alpha B subunit |
| Molecular Weight(MW) | 48737 Da |
| Function | CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Isoform AML-1L interferes with the transactivation activity of RUNX1. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the mouse BLK promoter. Inhibits KAT6B- dependent transcriptional activation |
| Tissue Specificity | Expressed in all tissues examined except brain and heart. Highest levels in thymus, bone marrow and peripheral blood |
| Cellular Localization | Nucleus. |
| Entrez Gene | 861 |
|---|
Application
A. Western blot analysis on (A) Molt-4 and (B) fetal thymus lysates using anti-RUNX1 RabMAb (Cat. #AJ1701a), dilution 1:10,000.
Application
B. Immunohistochemical analysis of paraffin-embedded human tonsils using anti-RUNX1 RabMAb (Cat. #AJ1701a).| Application Notes | WB:1:500~1000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
