Application
RUNX3 Antibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the RUNX3 antibody detected the RUNX3 protein (arrow).Application
RUNX3 Antibody (Center) (AP14667c)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RUNX3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Fluorescent confocal image of Hela cell stained with RUNX3 Antibody (Center)(Cat#AP14667c).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with RUNX3 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). RUNX3 immunoreactivity is localized to Nucleus and Cytoplasm significantly.| Product Name | RUNX3 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RUNX3; AML2; CBFA3; PEBP2A3; Runt-related transcription factor 3; Acute myeloid leukemia 2 protein; Core-binding factor subunit alpha-3; Oncogene AML-2; Polyomavirus enhancer-binding protein 2 alpha C subunit; SL3-3 enhancer factor 1 alpha C subunit; SL3/A |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | AML2 CBFA3 PEBP2A3 |
|---|---|
| Alternative Names | RUNX3 AML2 CBFA3 PEBP2A3 Runt-related transcription factor 3 Acute myeloid leukemia 2 protein Core-binding factor subunit alpha-3 Oncogene AML-2 Polyomavirus enhancer-binding protein 2 alpha C subunit SL3-3 enhancer factor 1 alpha C subunit SL3/A |
| Molecular Weight(MW) | 44356 Da |
| Function | CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, lck, IL-3 and GM-CSF promoters |
| Tissue Specificity | This RUNX3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 167-197 amino acids from the Central region of human RUNX3. |
| Cellular Localization | Nucleus. Cytoplasm. Note=The tyrosine phosphorylated form localizes to the cytoplasm |
| Entrez Gene | 864 |
|---|---|
| Nucleotide Accession | NP_001026850.1 NP_004341.1 |
Application
RUNX3 Antibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the RUNX3 antibody detected the RUNX3 protein (arrow).
Application
RUNX3 Antibody (Center) (AP14667c)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RUNX3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Fluorescent confocal image of Hela cell stained with RUNX3 Antibody (Center)(Cat#AP14667c).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with RUNX3 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). RUNX3 immunoreactivity is localized to Nucleus and Cytoplasm significantly.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
