Application
Western blot analysis of RXRA Antibody (Center) in MCF-7 cell line lysates (35ug/lane). RXRA (arrow) was detected using the purified Pab.Application
RXRA Antibody (Center) IHC analysis in formalin fixed and paraffin embedded lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RXRA Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | RXRA Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RXRA; NR2B1; Retinoic acid receptor RXR-alpha; Nuclear receptor subfamily 2 group B member 1; Retinoid X receptor alpha |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | NR2B1 |
|---|---|
| Alternative Names | RXRA NR2B1 Retinoic acid receptor RXR-alpha Nuclear receptor subfamily 2 group B member 1 Retinoid X receptor alpha |
| Molecular Weight(MW) | 50811 Da |
| Function | Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. The high affinity ligand for RXRs is 9-cis retinoic acid. RXRA serves as a common heterodimeric partner for a number of nuclear receptors The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone acetylation, chromatin condensation and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. The RXRA/PPARA heterodimer is required for PPARA transcriptional activity on fatty acid oxidation genes such as ACOX1 and the P450 system genes |
| Tissue Specificity | Highly expressed in liver, also found in lung, kidney and heart |
| Cellular Localization | Nucleus. |
| Entrez Gene | 6256 |
|---|
Application
Western blot analysis of RXRA Antibody (Center) in MCF-7 cell line lysates (35ug/lane). RXRA (arrow) was detected using the purified Pab.
Application
RXRA Antibody (Center) IHC analysis in formalin fixed and paraffin embedded lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RXRA Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
