Application
Western blot analysis of anti-SAE1 Pab in mouse lung tissue lysate. SAE1 (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.Application
SAE1 Antibody (V281) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the SAE1 antibody detected the SAE1 protein (arrow).Application
Western blot analysis of SAE1 (arrow) using rabbit polyclonal SAE1 Antibody (V281) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SAE1 gene.| Product Name | SAE1 (AOS1) Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SAE1; AOS1; SUA1; UBLE1A; SUMO-activating enzyme subunit 1; Ubiquitin-like 1-activating enzyme E1A |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | AOS1 SUA1 UBLE1A |
|---|---|
| Alternative Names | SAE1 AOS1 SUA1 UBLE1A SUMO-activating enzyme subunit 1 Ubiquitin-like 1-activating enzyme E1A |
| Molecular Weight(MW) | 38450 Da |
| Function | The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2 |
| Tissue Specificity | Expression level increases during S phase and drops in G2 phase (at protein level) |
| Cellular Localization | Nucleus. |
| Entrez Gene | 10055 |
|---|
Application
Western blot analysis of anti-SAE1 Pab in mouse lung tissue lysate. SAE1 (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Application
SAE1 Antibody (V281) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the SAE1 antibody detected the SAE1 protein (arrow).
Application
Western blot analysis of SAE1 (arrow) using rabbit polyclonal SAE1 Antibody (V281) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SAE1 gene.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
